Visual Peptide

Reconstitution & Dosing Guide

Basic steps for reconstituting lyophilized peptides and calculating doses. For research use only.

First time here? Read sections 1 → 2 → 3 → 4 in order for the full workflow.

1. What you need

  • Lyophilized peptide vial (e.g. 5 mg)
  • Bacteriostatic water (BAC water) or sterile diluent per protocol
  • Syringe (e.g. 1 mL insulin syringe for dosing)
  • Clean, sterile technique

2. Units you’ll see

Doses and labels use standard mass and volume units. Converting correctly avoids errors.

  • mg (milligram) — peptide per vial, e.g. 5 mg.
  • mcg (microgram) — dose per injection; 1000 mcg = 1 mg.
  • mL (milliliter) — diluent volume and volume to draw; 1 mL = 1000 µL.
  • IU — sometimes used for certain peptides (e.g. some GLP-1); convert using product or literature when needed.

For quick mcg ↔ mg and other conversions, use our Unit Converter.

3. Reconstitution steps

  1. Let vial and diluent reach room temperature if stored cold.
  2. Draw the desired volume of diluent into a syringe.
  3. Inject the diluent slowly into the peptide vial (along the wall to avoid foaming).
  4. Gently swirl or roll — do not shake — until the powder is fully dissolved.
  5. Store reconstituted peptide as per protocol (often 2–8 °C and use within a defined period).

To see exactly where to draw to on your syringe after reconstituting, use the Syringe Planner.

4. Concentration and dose

Concentration (mg/mL) = peptide mass (mg) ÷ diluent volume (mL). To hit a target dose in mcg: convert dose to mg (dose ÷ 1000), then divide by concentration to get the volume (mL) to draw. Use our Recon & Dosing Calculator for exact numbers and syringe units.

Doses cited here and in our peptide pages are for research reference only; clinical use follows different protocols and regulations.

5. Purity and sourcing

Peptide identity and purity should be verified by third-party testing (e.g. HPLC, mass spec). Before you source, use our Purity & Verify page to check reports (e.g. Janoshik Task ID) and see the public result database.

6. Reconstitution stability / use-by

Many peptides are stable 2–8 °C for about 30 days after reconstitution. Stability varies by peptide and formulation; the 30-day default is a common reference only. Follow product or protocol.

For reference only. Follow manufacturer or protocol for storage and stability.

7. Tools at a glance

Use the right tool for each step of your workflow:

  • Recon & Dosing Calculator — Peptide mass (mg), diluent (mL), target dose (mcg), syringe size → concentration, volume to draw, units. Use after recon to plan each injection.
  • Syringe Planner — Same inputs as the calculator plus syringe type → visual syringe showing where to draw to. Use when you want to see the fill line.
  • Vial & Cycle — Dose, frequency, vial size → days per vial, vials per cycle. Use to plan how many vials to buy and how long one vial lasts.
  • Unit Converter — mcg ↔ mg and other mass/volume conversions. Use for quick unit checks.
  • Cost per Dose — Price per vial, mg per vial, dose (mcg) → cost per injection. Use to compare cost across products or doses.

For peptide reference (typical dose, frequency, recon notes): Peptide Library. To compare 2–3 peptides side by side: Compare. For 3D structure: 3D Structure.

8. More questions

Common questions about reconstitution, storage, and verification are on our FAQ.